a study of the efficiency of some serological and pcr -based methods for the identification and detection of erwinia amylovora, the causal agent of pome trees fire blight

نویسندگان

ناهید معرف زاده

مجتبی محمدی

عباس شریفی تهرانی

زهرا ذاکری

چکیده

fire blight, a bacterial disease caused by erwinia amylovora (burr.) winslow et al. 1920 is considered as a serious disease on pome fruits. during recent years, this disease has caused serious damages to the fruit growing industry in iran. precise and reliable methods for identification and detection of the pathogen can help in its early control, prevent the disease spread and help eliminate the infected trees in orchards and particularly young trees in the nurseries. in this study, two specific polyclonal antisera were developed and used in double diffusion in agar test and dot immunobinding assay (diba) to detect e. amylovora (ea) cells. as many as 1×107 cells per ml from pure culture and as well from infected tissues were detected through diba. the sensitivity level was increased to as low as 10 cells using the bacterial enrichment step. the efficiency of serological methods was compared with some published pcr based identification methods, too, in which, a single 1kb dna fragment (psti) of pea29 plasmid was amplified in polymerase chain reaction (pcr) by using a and b primer pair. the sensitivity levels of this method in water and tissue extract were 2×104 vs 2×106 cfu/reaction mixture, respectively. the three mentioned methods were able to identify ea strains representing various hosts and regions of the country and were specific for ea, in contrast with other bacterial genera. in nested-pcr, aj75 and aj76 specific primers were used to amplify a single 800- bp fragment. in each reaction mixture, this highly sensitive method could detect as few as one and as many as 100 ea cells in water and in tissue extract, respectively.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

wuthering heights and the concept of marality/a sociological study of the novel

to discuss my point, i have collected quite a number of articles, anthologies, and books about "wuthering heights" applying various ideas and theories to this fantastic story. hence, i have come to believe that gadamer and jauss are rightful when they claim that "the individaul human mind is the center and origin of all meaning," 3 that reading literature is a reader-oriented activity, that it ...

15 صفحه اول

a case study of the two translators of the holy quran: tahereh saffarzadeh and laleh bakhtiar

بطورکلی، کتاب های مقدسی همچون قران کریم را خوانندگان میتوان مطابق با پیش زمینه های مختلفی که درند درک کنند. محقق تلاش کرده نقش پیش زمینه اجتماعی-فرهنگی را روی ایدئولوژی های مترجمین زن و در نتیجه تاثیراتش را روی خواندن و ترجمه آیات قرآن کریم بررسی کند و ببیند که آیا تفاوت های واژگانی عمده ای میان این مترجمین وجود دارد یا نه. به این منظور، ترجمه 24 آیه از آیات قرآن کریم مورد بررسی مقایسه ای قرار ...

15 صفحه اول

study of cohesive devices in the textbook of english for the students of apsychology by rastegarpour

this study investigates the cohesive devices used in the textbook of english for the students of psychology. the research questions and hypotheses in the present study are based on what frequency and distribution of grammatical and lexical cohesive devices are. then, to answer the questions all grammatical and lexical cohesive devices in reading comprehension passages from 6 units of 21units th...

formation and evolution of regional organizations: the case study of the economic cooperation organization (eco)

abstract because of the many geopolitical, geo economical and geo strategically potentials and communicational capabilities of eco region, members can expand the convergence and the integration in base of this organization that have important impact on members development and expanding peace in international and regional level. based on quality analyzing of library findings and experts interv...

15 صفحه اول

the study of practical and theoretical foundation of credit risk and its coverage

پس از بررسی هر کدام از فاکتورهای نوع صنعت, نوع ضمانت نامه, نرخ بهره , نرخ تورم, ریسک اعتباری کشورها, کارمزد, ریکاوری, gdp, پوشش و وثیقه بر ریسک اعتباری صندوق ضمانت صادرات ایران مشخص گردید که همه فاکتورها به استثنای ریسک اعتباری کشورها و کارمزد بقیه فاکتورها رابطه معناداری با ریسک اعتباری دارند در ضمن نرخ بهره , نرخ تورم, ریکاوری, و نوع صنعت و ریسک کشورها اثر عکس روی ریسک اعتباری داردو پوشش, وثی...

15 صفحه اول

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
دانش گیاه پزشکی ایران

جلد ۴۰، شماره ۱، صفحات ۰-۰

کلمات کلیدی
fire blight a bacterial disease caused by erwinia amylovora (burr.) winslow et al. 1920 is considered as a serious disease on pome fruits. during recent years this disease has caused serious damages to the fruit growing industry in iran. precise and reliable methods for identification and detection of the pathogen can help in its early control prevent the disease spread and help eliminate the infected trees in orchards and particularly young trees in the nurseries. in this study two specific polyclonal antisera were developed and used in double diffusion in agar test and dot immunobinding assay (diba) to detect e. amylovora (ea) cells. as many as 1×107 cells per ml from pure culture and as well from infected tissues were detected through diba. the sensitivity level was increased to as low as 10 cells using the bacterial enrichment step. the efficiency of serological methods was compared with some published pcr based identification methods too in which a single 1kb dna fragment (psti) of pea29 plasmid was amplified in polymerase chain reaction (pcr) by using a and b primer pair. the sensitivity levels of this method in water and tissue extract were 2×104 vs 2×106 cfu/reaction mixture respectively. the three mentioned methods were able to identify ea strains representing various hosts and regions of the country and were specific for ea in contrast with other bacterial genera. in nested pcr aj75 and aj76 specific primers were used to amplify a single 800 bp fragment. in each reaction mixture this highly sensitive method could detect as few as one and as many as 100 ea cells in water and in tissue extract respectively.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023